Selection of mandelamidase constitutive mutants in continuous culture ofPseudomonas putida |
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| Authors: | Peter D. Laverack Patricia H. Clarke |
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| Affiliation: | (1) Department of Biochemistry, University College London, WC1E 6BT London |
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| Abstract: | Summary A method is described for the isolation of mandelamidase constitutive mutants ofPseudomonas putida in continuous culture. Growth was nitrogen-limited and the nitrogen was provided either as inducing substrate (mandelamide) or non-inducing substrate (isobutyramide) alternately. |
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