ras和c-erbB癌基因在特发性肺间质纤维化中的表达

采用原位分子杂交技术和免疫组织化学方法，对８例临床与肺组织学确诊的特发性肺间质纤维化（ＩＰＦ）病人的肺活检组织及７例正常肺组织进行了ｒａｓ和ｃ－ｅｒｂＢ癌基因及其产物的检测。结果：ｒａｓ癌基因产物Ｐ２１在６／８例ＩＰＦ肺组织中呈阳性反应；５／８例ＩＰＦ肺组织ｃ－ｅｒｂＢ－２显免疫阳性反应，而在７例正常肺组织Ｐ２１及ｃ－ｅｒｂＢ－２均无明显表达，原位杂交结果显示ＩＰＦ肺组织没有明显ｒａｓ和ｃ－ｅｒｂＢ癌基因的ＤＮＡ扩增提示ＩＰＦ的病变过程与ｒａｓ和ｃ－ｅｒｂＢ癌蛋白表达增强有关

EXPRESS OF P ras 21 AND c erbB 2 IN IDIOPATHIC PULMONARY FIBROSIS

Eight pulmonary bioptic tissues with idiopathic pulmonary fibrosis (IPF) that have been diagnosed by clinical and pulmonary histology and seven normal lung tissues were stained by immunohistochemistry LSAB method with monoclonal antibodies to P ras 21 and c erbB 2 and by hybridization with ras and c erbB cDNA probe. THe results showed that immunostaining of P 21 was found in 6/8 cases of IPF, and c erbB 2 immunoactivity staining was shown in 5/8 cases of IPF. But the immunostaining of the two autibodies was negative in all normal pulmonary tissues. Positive hybridization staining was observed only in a minority of cells in IPF pulmonary tissue. There is no difference between the IPF and normal groups. This result suggests that increase of oncoprotein P ras 21 and c erbB 2 might be associated with pathogenesis of IPF.