Nuclear DNA fragmentation during cell death of short-lived ray tracheids in the conifer <Emphasis Type="Italic">Pinus densiflora</Emphasis> |
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Authors: | Satoshi Nakaba Takafumi Kubo Ryo Funada |
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Institution: | (1) Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-Tokyo 183-8509, Japan; |
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Abstract: | One key event in the programmed cell death is nuclear DNA fragmentation. We investigated the timing of nuclear DNA fragmentation
during the cell death of short-lived ray tracheids in Pinus densiflora using the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Fluorescence due to TUNEL
was detected only in deformed nuclei that lacked obvious chromatin in ray tracheids that were adjacent to ray tracheids that
no longer contained nuclei. Our observations revealed that nuclear DNA fragmentation occurred only at the final stage of cell
death in ray tracheids in situ. |
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