An efficient genotype-independent method for regeneration of potato plants from leaf tissue |
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Authors: | John S Hulme Elaine S Higgins Robert Shields |
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Institution: | (1) Plant Breeding International Cambridge, Maris Lane, Trumpington, CB2 2LQ Cambridge, UK |
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Abstract: | The regeneration of plants from leaf explants of a number of potato cultivars using a number of published one-, two- and three-step methods was assessed. A method using a pretreatment with high levels of auxin and cytokinin coupled with silver thiosulphate in the regeneration medium proved the most rapid and efficient for the eight cultivars examined.Abbreviations BA
benzyladenine
- GA3
gibberellic acid
- IAA
indoleacetic acid
- MS
Murashige and Skoog
- NAA
naphthaleneacetic acid
- STS
silver thiosulphate |
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Keywords: | tissue culture Solanum tuberosum |
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