Absence of clustering of phosphatidylinositol-(4,5)-bisphosphate in fluid phosphatidylcholine |
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Authors: | Fernandes Fábio Loura Luís M S Fedorov Alexander Prieto Manuel |
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Institution: | Centro de Química-Física Molecular, Instituto Superior Técnico, Lisbon, Portugal. fernandesf@ist.utl.pt |
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Abstract: | Phosphatidylinositol-(4,5)-bisphosphate PI(4,5)P(2)] plays a key role in the modulation of actin polymerization and vesicle trafficking. These processes seem to depend on the enrichment of PI(4,5)P(2) in plasma membrane domains. Here, we show that PI(4,5)P(2) does not form domains when in a fluid phosphatidylcholine matrix in the pH range of 4.8-8.4. This finding is at variance with the spontaneous segregation of PI(4,5)P(2) to domains as a mechanism for the compartmentalization of PI(4,5)P(2) in the plasma membrane. Water/bilayer partition of PI(4,5)P(2) is also shown to be dependent on the protonation state of the lipid. |
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Keywords: | PIP2 lipid domains fluorescence fluorescence resonance energy transfer |
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