Stimulated secretion of lysosomal enzymes induced by drugs in transimmortalized proximal tubule mouse kidney cells |
| |
| Authors: | A. Vandewalle |
| |
| Affiliation: | (1) Institut Fédératif de Recherche, Faculté de Médecine Xavier Bichat, Institut National de la Santé et de la Recherche Médicale, INSERM U246, Paris, France;(2) Faculté de Médicine Xavier Bichat, INSERM U246, Cedex 18, B.P. 416, 75870 Paris, France |
| |
| Abstract: | We summarize the results of study of the properties of two models of transimmortalized proximal tubule epithelial cells derived from the kidneys of transgenic mice harboring the SV40 large T and little t antigens/L-pyruvate kinase hybrid gene. The two cell lines, reffered to as PKSV-PCT and PKSV-PR cells, maintained for long-term passages the main biochemical and functional properties from the convoluted and terminal parts of the proximal tubule, respectively from which they were derived. In PKSV-PCT cells, gentamicin induced lysosomal alkalinization, decreased the cellular N-acetyl- -D-glucuronidase, and stimulated its secretion in a dose-dependent manner. The results indicate that these models of mouse proximal cultured cells could be suitable models for the study of the cellular action of drugs.Abbreviations MDR multidrug resistance - NAG N-acetyl--D-glucuronidase - PGP P-glycoprotein |
| |
| Keywords: | aminoside cell culture kidney lysosomal enzymes proximal tubule |
| 本文献已被 SpringerLink 等数据库收录！ |
|
