Acquisition of a potential marker for insect transformation: isolation of a novel alcohol dehydrogenase gene from Bactrocera oleae by functional complementation in yeast |
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| Authors: | Benos P Tavernarakis N Brogna S Thireos G Savakis C |
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| Institution: | (1) Institute of Molecular Biology and Biotechnology, FORTH, PO Box 1527, Heraklion GR-71110, Crete, Greece, GR;(2) Division of Medical Sciences, Medical School, University of Crete, Heraklion, Crete, Greece, GR;(3) Dept. of Genetics, Campus Box 8232, Washington University, School of Medicine, 4566 Scott Avenue, St. Louis, MO 63110, USA E-mail: benos@genetics.wustl.edu Tel.: +1-314-7475535; Fax: +1-314-3627855, US |
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| Abstract: | The alcohol dehydrogenase genes make up one of the best studied gene families in Drosophila, both in terms of expression
and evolution. Moreover, alcohol dehydrogenase genes constitute potential versatile markers in insect transformation experiments.
However, due to their rapid evolution, these genes cannot be cloned from other insect genera by DNA hybridization or PCR-based
strategies. We have therefore explored an alternative strategy: cloning by functional complementation of appropriate yeast
mutants. Here we report that two alcohol dehydrogenase genes from the medfly Ceratitis capitata can functionally replace the yeast enzymes, even though the medfly and yeast genes have evolved independently, acquiring
their enzymatic function convergently. Using this method, we have cloned an alcohol dehydrogenase gene from the olive pest
Bactrocera oleae. We conclude that functional complementation in yeast can be used to clone alcohol dehydrogenase genes that are unrelated
in sequence to those of yeast, thus providing a powerful tool for isolation of dominant insect transformation marker genes.
Received: 29 June 1999 / Accepted: 27 October 1999 |
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| Keywords: | Drosophila melanogaster Ceratitis capitata Tephritids Evolution Dacus |
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