Method for extraction of proteins from green plant tissues for two-dimensional polyacrylamide gel electrophoresis |
| |
| Authors: | F Cremer C Van de Walle |
| |
| Affiliation: | 1. Molecular and Structural Biology Division, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India;2. Division of Parasitology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh 226031, India;3. King George Medical University, Lucknow, Uttar Pradesh 226003, India |
| |
| Abstract: | A method has been developed for the extraction of proteins from green plant tissues to be used in two-dimensional polyacrylamide gel electrophoresis. Three purification steps were necessary to overcome the problems due to streaking, charge heterogeneity, and other artifacts: After it was ground in liquid nitrogen, the powdered material was stirred in the presence of insoluble polyvinylpyrrolidone for binding phenols, and sodium ascorbate for binding quinones; proteins were precipitated with ammonium sulfate, and the sample was dialyzed. Hundreds of proteins could be detected after Coomassie blue staining using 200 micrograms of proteins from apical buds of Sinapis alba L. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
