Detection of DNA damage in spermiogenic stages of mice treated with enriched uranyl fluoride by alkaline elution

Summary DNA breakage in spermiogenic stages of mice treated with enriched uranyl fluoride (UO₂F₂) was studied using an alkaline elution technique. Mature spermatozoa were sampled from the animal's vas and eluted with a buffer (PH 12.2) at 3-day intervals over a 33-day period after i.p. injection of 2 mg U0₂F₂/kg and always at 36 days after thymidine labeling in the testes. Elution of sperm DNA from treated animals varied with spermiogenic stages. At 12 days after exposure the amount of the elution of sperm DNA was found highest and increased with the increasing U0₂F₂ dose up to 6 mg/kg.