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Oxygen-bound Hell's gate globin I by classical versus LB nanotemplate method
Authors:Pechkova Eugenia  Scudieri Dora  Belmonte Luca  Nicolini Claudio
Affiliation:Nanobiotechnology and Biophysics Laboratories, Department Experimental Medicine, University of Genova, Genova, Italy.
Abstract:X‐ray atomic structure of recombinant Hell's gate globin I (HGbI) from Methylacidophilum infernorum was calculated from the X‐ray diffraction data of two different types of crystals: obtained by classical hanging drop and by LB nanotemplate method under the same crystallization conditions. After the accurate comparison of crystallographic parameters and electron density maps of two structures they appears to be quite similar, while the quality of the crystals grown by LB nanotemplate method was higher then of those grown by classical method. Indeed, the resolution of the LB crystal structure was 1.65 Å, while classical crystals showed only 3.2 Å resolution. Moreover, the reproducibility of this result in the case of LB crystals was much better—nine crystals from 10 gave the same structural results, while only two of 10 classical crystals were appropriate for the X‐ray structure resolution. J. Cell. Biochem. 113: 2543–2548, 2012. © 2012 Wiley Periodicals, Inc.
Keywords:HELL'S GATE GLOBIN I  THIN FILM  LANGMUIR BLODGETT
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