Somatic Embryogenesis and Bud Formation on Cultured Fagopyrum esculentum Hypocotyls |
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Authors: | Gumerova E A Galeeva E I Chuyenkova S A Rumyantseva N I |
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Institution: | (1) Kazan Institute of Biochemistry and Biophysics, Kazan Research Center, Russian Academy of Sciences, ul. Lobachevskogo 2/31, Kazan, Tatarstan, 420503, Russia |
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Abstract: | An indirect somatic embryogenesis via the development of proembryogenic cell complexes (PECC) was observed in the in vitro cultured hypocotyl explants of 4–5-day-old buckwheat (Fagopyrum esculentum Moench.) seedlings. PECC development was shown to depend on culturing conditions, including 2,4-D concentration and the period of explant exposure to 2,4-D, sucrose concentration, and explant density. The culturing protocol was designed to ensure the development of buckwheat somatic embryos in two-month period. The cytogenetic analysis demonstrated that this protocol did not affect the chromosome numbers in the regenerated plants. |
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Keywords: | Fagopyrum esculentum cell culture somatic embryogenesis proembryogenic cell complexes hypocotyl |
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