Monomeric A beta and metals reduce their cytotoxicities to each other |
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| Authors: | Matsuzaki Shinsuke Yasuda Yuichi Kobayashi Shinya Koyama Yoshihisa Kawamoto Keisuke Katayama Taiichi Tohyama Masaya |
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| Institution: | Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka, Japan. s-matsuzaki@anat2.med.osaka-u.ac.jp |
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| Abstract: | The present study has examined the effect of metals, such as iron and copper on the cytotoxicity of amyloid beta protein 1-40 (Abeta40). First, we showed that monomeric Abeta40 has stronger cytotoxicity than various type of aggregated Abeta40. Next we showed the addition of metals into the monomeric Abeta40 reduced the cytotoxicity of either monomeric Abeta40 or metals (iron and copper) although the addition of metals into monomeric Abeta40 resulted in a marked increase of aggregated form of Abeta40, which composed of beta-sheeted Abeta40 and Abeta40 aggregation not characterized by beta-sheet fibrils (coagrated Abeta40). Taken together, the metals and monomeric Abeta40 affect on each other and cause the reduction of their cell toxicity. |
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| Keywords: | Monomeric Aβ40 Aβ40 which monomeraized by HFPI and dissolved to PBS Coagrated Aβ40 the aggregation forms of Aβ40 which are not characterized by β-sheet fibrils Total-aggregation (T-aggregation) all of aggregation forms of Aβ40 which include coagrated and β-sheeted Aβ40 Agg1 aged-Aβ40 used as a seed Agg2 Aβ40 solution which is made by the incubation of monomeric Aβ40 for 120 h Agg3 Aβ40 solution which is made by the incubation of monomeric Aβ40 with aged-Aβ for 120 h |
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