| 尾叶桉的不同类型愈伤组织芽分化与某些相关生理生化指标的关系 |
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| 引用本文： | 黄真池,欧阳乐军,曾富华,余秋梅.尾叶桉的不同类型愈伤组织芽分化与某些相关生理生化指标的关系[J].植物生理学通讯,2010(2):147-149. |
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| 作者姓名： | 黄真池 欧阳乐军 曾富华 余秋梅 |
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| 作者单位： | 湛江师范学院生命科学与技术学院; |
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| 基金项目： | 广东省自然科学基金(8152404801000011);;广东省科技攻关项目(2006B20101011) |
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| 摘 要： | 切取8d苗龄的尾叶桉幼苗下胚轴,培养7d后愈伤组织的诱导率达100%。8周后,愈伤组织分化成褐色、白色和浅绿色3种类型。其中只有浅绿色愈伤组织能分化出不定芽,分化率达57%。浅绿色愈伤组织的过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和多酚氧化酶（PPO）的活性最高。除SOD外,浅绿色愈伤组织的POD、CAT和PPO活性与其他两类愈伤组织相比具有显著性差异（P〈0.05）。H2O2和丙二醛含量则是褐色愈伤组织的最高。
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| 关 键 词： | 尾叶桉 愈伤组织 过氧化物酶 过氧化氢酶 |
Relationship between Bud Differentiation and Several Physiological and Biochemical Indexes of Three Types of Eucalyptus urophylla S.T.Blake Callus |
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| HUANG Zhen-Chi,OUYANG Le-Jun,ZENG Fu-Hua,YU Qiu-Mei Life Science & Technology School,Zhanjiang Normal University,Zhanjiang,Guangdong ,China.Relationship between Bud Differentiation and Several Physiological and Biochemical Indexes of Three Types of Eucalyptus urophylla S.T.Blake Callus[J].Plant Physiology Communications,2010(2):147-149. |
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| Authors: | HUANG Zhen-Chi OUYANG Le-Jun ZENG Fu-Hua YU Qiu-Mei Life Science & Technology School Zhanjiang Normal University Zhanjiang Guangdong China |
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| Institution: | HUANG Zhen-Chi,OUYANG Le-Jun,ZENG Fu-Hua,YU Qiu-Mei Life Science & Technology School,Zhanjiang Normal University,Zhanjiang,Guangdong 524048,China |
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| Abstract: | Hypocotyls excised from 8 day-old seedlings could dedifferentiate and form callus at 100% when they were incubated on a modified MS (SPCa) medium for 7 days.Fifty-six days later,the callus differentiated into 3 types,including brown callus,white callus and reseda callus.Only could the reseda callus formed adventitious bud at 57% when these calli inoculated with the bud induction medium.The activities of POD,SOD,CAT and PPO of reseda callus were the highest.Compared with the enzyme activity of other types of... |
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| Keywords: | Eucalyptus urophylla callus peroxidase catalase |
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