Direct observation of fat crystallization in a living fly by X-ray diffraction: fat crystallization does not cause the fly's instantaneous death, but ice formation does |
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| Authors: | Takahashi Hiroshi Katagiri Chihiro Ueno Satoru Inoue Katsuaki |
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| Institution: | aBiophysics Laboratory, Department of Chemistry and Chemical Biology, Graduate School of Engineering, Gunma University, 4-2, Aramaki, Maebashi 371-8510, Japan;bBiochemistry Laboratory, Institute of Low Temperature Science, Hokkaido University, Japan;cGraduate School of Biosphere Science, Hiroshima University, Japan;dJapan Synchrotron Radiation Research Institute (SPring-8/JASRI), Japan |
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| Abstract: | A cool-temperate fly, Drosophila triauraria, stores fat, triacylglycerol (TAG), primarily in the fat storage organ, the fat body, and then diapauses to pass the winter in imago stage. TAG crystallization and ice formation taking place in a living fly by lowering temperatures were studied, in order to clarify the relationship between crystallizations and the fly’s death at lower temperatures. X-ray diffraction, a direct non-invasive method, was used to detect the liquid-to-crystal transformations of TAG and water. During cooling, TAG crystallization preceded ice formation. It was also found that ice formation causes the fly to die instantaneously whereas the TAG crystallization does not. |
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| Keywords: | Drosophila fly Overwintering Triacylglycerol Ice Crystallization X-ray diffraction |
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