Comparison of Five Media for the Isolation of Coliform Organisms from Dehydrated and Deep Frozen Foods |
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Authors: | R. S. Moussa Nicole Keller G. Curiat J. C. De Man |
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Affiliation: | Control Laboratory, NestléProducts Technical Assistance Company, La Tour de Peilz, Switzerland |
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Abstract: | S ummary . A significantly higher number of coliform and faecal coliform organisms were isolated using lactose-glutamic acid medium than by using lauryl sulphate-tryptone, lactose, brilliant green-bile or EE broths. With brilliant green-bile and lauryl sulphate-tryptone broth, coliforms/Enterobacteriaceae were isolated less often than with the others. For the detection of faecal coliforms, EE broth proved less good than all other media. All 5 media showed more coliforms/Enterobacteriaceae after 48 h than after 24 h at 30°. Few false positive results were obtained using any of these media. |
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