An improved method for the isolation of total RNA from spurce tissues |
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| Authors: | Mouna Bahloul Gérard Burkard |
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| Institution: | (1) Institute de Biologie Moléculaire des Plantes, 12 rue du général Zimmer, 67084 Strasbourg Cedex, France |
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| Abstract: | After many unsuccessful attempts to obtain biologically active mRNAs from spruce (Picea abies) tissues using available protocols, we have adapted a procedure for the isolation of RNAs from needles, shoots, and callus
ofPicea species. Our modifications permit the recovery of and an average of 300 μg RNA per g of needles that is suitable for translationin vitro, northern hybridizations, and the construction of cDNA libraries. |
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| Keywords: | mRNA purification Picea abies spruce tissues |
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