Degradation of 2-Methylnaphthalene by Pseudomonas sp. Strain NGK1 |
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Authors: | U Sharanagouda TB Karegoudar |
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Institution: | (1) Department of Biochemistry, Gulbarga University, Gulbarga-585 106, India, IN |
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Abstract: | Pseudomonas sp. strain NGK1, a soil bacterium isolated by naphthalene enrichment from biological waste effluent treatment, capable of
utilizing 2-methylnaphthalene as sole source of carbon and energy. To deduce the pathway for biodegradation of 2-methylnaphthalene,
metabolites were isolated from the spent medium and identified by thin-layer chromatography and high-performance liquid chromatography.
The characterization of purified metabolites, oxygen uptake studies, and enzyme activities revealed that the strain degrades
2-methylnaphthalene through more than one pathway. The growth of the bacterium, utilization of 2-methylnaphthalene, and 4-methylsalicylate
accumulation by Pseudomonas sp. strain NGK1 were studied at various incubation periods.
Received: 20 March 2001 / Accepted: 25 April 2001 |
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