Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis |
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| Authors: | D. P. Sartory J. U. Grobbelaar |
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| Affiliation: | (1) Department of Environment Affairs, Hydrological Research Institute, Private Bag X313, 0001 Pretoria, Republic of South Africa;(2) Institute for Environmental Sciences, University of the Orange Free State, P.O. Box 339, 9300 Bloemfontein, Republic of South Africa |
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| Abstract: | The efficiency of pigment extraction forms the crux of the spectrophotometric analysis of chlorophyll a. The alcoholic solvents, methanol and ethanol, proved to be superior to acetone and acetone with DMSO. Homogenisation and sonication did not improve the extraction in the alcoholic solvents. Boiling at 100°C had an adverse effect whereas complete extraction of the pigments was obtained at the solvents boiling point and allowing the samples to stand for 24 h in the dark. |
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| Keywords: | chlorophyll a extraction solvents homogenisation boiling sonication |
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