洋葱花粉发育过程中ATP酶的分布特征(简报)

在真核细胞中,除了线粒体和叶绿体ATPase的功能是合成ATP外,其余部位ATPase是水解ATP以获取生物能量的代谢酶,在生物体细胞内广泛存在。探索ATPase在细胞中的分布状态是研究细胞生理状态的一种重要手段。ATPase在细胞中的多少可反映出细胞当时的生活状态,这一特征已被初步用于探索小麦和水稻雄性不育的细胞生物学研究中,希望通过比较可育花药和不育花药中ATPase的分布差异寻找雄性不育的机理,发现

THE DISTRIBUTION OF ATPase DURING THE POLLEN DEVELOPMENT OF ALLIUM CEPA L.

The distribution of ATPase in pollen of Allium cepa L.was studied using Pb_3 precipitation technique during pollen development.Only some ATPase precipitates were located in the nucleus of microspore mother cells(MMC)and a few in its cytoplasm.After meiosis of MMC,many ATPase precipitates appeared in the exine of pollen wall of microspore even it was in tetrad,suggesting that ATPase from tapetum is necessary during pollen wall construction.The intine of pollen wall of microspore was synthesized at its late stage and consisted of cellular material which was from microspore.There were also many ATPase precipitates in intine,and the ATPase came from microspore.Then ATPase precipitates in vegetative cell increased and that in generative cell decreased during the development of 2-cellular pollen,suggesting the differentiation of vigor between both cells.The physiological functions of ATPase in developing pollen of Allium cepa L.were analyzed.