Molecular cloning of Bacillus subtilis genes involved in DNA metabolism |
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| Authors: | Marta Perego Eugenio Ferrari Maria Teresa Bassi Alessandro Galizzi and Piergiorgio Mazza |
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| Institution: | (1) Dipartimento di Genetica e Microbiologia  A. Buzzati-Traverso , Università di Pavia, I-27100 Pavia, Italy;(2) Present address: Scripps Clinic and Research Foundation, 92037 La Jolla, CA, USA;(3) Present address: Genencor, Inc., 94080 South San Francisco, CA, USA;(4) Present address: Istituto di Tecnica Farmaceutica, Università di Parma, Via M. D'Azeglio 85, I-43100 Parma, Italy |
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| Abstract: | Summary Different clones carrying a chromosomal DNA fragment able to transform Bacillus subtilis mutants dnaA13, dnaB19, dnaG5, recG40 and polA42 to a wild-type phenotype were isolated from a library constructed in plasmid pJH101. A  recombinant clone carrying a chromosomal fragment able to transform dnaC mutants was obtained from a Charon 4A library. A restriction map of the cloned DNA fragments was constructed. The 11.3 kb cloned DNA fragment of plasmid pMP60-13 containing the wild-type sequence of dnaG5 was shown to transform a recF33 mutant as well. |
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| Keywords: | Bacillus subtilis DNA metabolism genes Gene cloning |
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