| 采用花器喷雾法并基于Cre/lox系统定点整合拟南芥Rps2基因的技术体系研究 |
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| 引用本文： | 李定红,张艳梅,周广灿,雷照,孙小芹,杭悦宇.采用花器喷雾法并基于Cre/lox系统定点整合拟南芥Rps2基因的技术体系研究[J].植物资源与环境学报,2017(1):104-106. |
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| 作者姓名： | 李定红 张艳梅 周广灿 雷照 孙小芹 杭悦宇 |
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| 作者单位： | 江苏省中国科学院植物研究所(南京中山植物园) ,江苏 南京,210014 |
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| 基金项目： | 江苏省科技计划项目(省属公益类科研院所能力提升)(BM2015019)，江苏省自然科学基金资助项目(BK2010476)，江苏省植物迁地保护重点实验室开放基金项目(迁201202)，江苏省中国科学院植物研究所青年基金项目(SQ201404) |
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| 摘 要： | To improve site?specific integration technology system, site?specific integration of Rps2 target gene in Arabidopsis thaliana (Linn.) Heynh. was carried out based on Cre/lox system by floral spraying method. The results show that 1495 site?specific integration candidate plants are obtained by this method with a site?specific integration efficiency of about 0076%. After PCR and histochemical staining experiment verification, the positive plants of precise integration account for 8604%, in which, 6334% positive plants are single copy transformed plants. The results of quantitative real?time PCR (qRT?PCR) and hypersensitive reaction (HR) show that the site?specific integrated Rps2 gene can be transcribed and expressed normally. It is suggested that this system can greatly improve the stability and efficiency of site?specific integration genetic transformation system in plants.
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| 关 键 词： | Cre/lox系统 Rps2基因 定点整合 拟南芥 花器喷雾法 |
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