Somatic embryogenesis and plantlet production using rejuvenated tissues from serial grafting of a mature <Emphasis Type="Italic">Kalopanax septemlobus</Emphasis> tree |
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Authors: | Heung Kyu Moon So Young Park Yong Wook Kim Sea Hyun Kim |
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Institution: | (1) Division of Biotechnology, Korea Forest Research Institute, Omokdong 44-3, Suwon, 441-350, South Korea;(2) Division of Special Tree Breeding, Korea Forest Research Institute, Omokdong 44-3, Suwon, 441-350, South Korea |
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Abstract: | An effective micropropagation technique via somatic embryogenesis has been developed using tissue from serially grafted shoots
generated from a mature Kalopanax septemlobus tree (~40 y old). Callus was induced from leaf segments obtained from the grafts by culturing the explants in Murashige and
Skoog (MS) medium supplemented with 2,4-D and 3% w/v sucrose under darkness. The effects of sucrose, coconut water, and polyethylene glycol (PEG-3350) were evaluated as factors
to promote development of somatic embryos (SEs) from embryogenic callus. More than 90% of explants formed callus; however,
only 2.5%, or 20 leaf segments out of 800 explants, formed embryogenic callus after 8 wk of culture. High sucrose concentrations
(3% and 5% w/v) were effective in inducing SEs. Treatment with 2–10% v/v coconut water also had a positive effect on embryo induction. A synergistic effect on SE induction was obtained using sucrose
and PEG, with presence of the latter compound resulting in smaller, more uniform SEs. Embryo germination and conversion to
plantlets were significantly influenced by the gelling agents. In general, gelrite-gelled medium was superior to agar-gelled
medium. In gelrite-gelled medium, gibberillic acid (GA3) enhanced embryo germination. Converted plantlets in an artificial soil mixture showed a 91% survival rate and displayed
no distinct morphological variations. Our results indicate that reliable somatic embryogenesis and plant production can be
achieved with rejuvenated tissues after repeated grafting of shoots derived from a mature Kalopanax septemlobus tree. |
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Keywords: | Kalopanax septemlobus Somatic embryogenesis Rejuvenation Grafting Coconut water Polyethylene glycol |
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