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BcWRKY1 confers salt sensitivity via inhibiting Reactive oxygen species scavenging |
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| Authors: | Yuan Shuilin Hu Die Wang Yuan Shao Cen Liu Tongkun Zhang Changwei Cheng Feng Hou Xilin Li Ying |
| Affiliation: | 1.National Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, Jiangsu Province, China ;2.Guangdong Key Laboratory of Tea Plant Resources Innovation & Utilization, Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, Guangdong Province, China ;3.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Beijing, China ; |
| Abstract: | WRKY transcription factors play important roles in abiotic stress by directly regulating stress-related genes. However, the molecular mechanism of its involvement in salt stress in pak-choi is still poorly understood. In this study, we elucidated the function of BcWRKY1 from pak-choi (Brassica rapa ssp. chinensis) in salt stress. The expression level of BcWRKY1 showed the highest in rosette leaves among different tissues and was induced by salt and ABA treatment in pak-choi. Subcellular localization showed that BcWRKY1 was located in nucleus. The transgenic Arabidopsis overexpressing BcWRKY1 exhibited enhanced salt sensitivity and higher H2O2 contents, which were further confirmed by silencing BcWRKY1 in pak-choi. In addition, the expression of ZAT12 was negatively regulated with BcWRKY1 under salt stress both in pak-choi and Arabidopsis. Yeast one-hybrid and dual luciferase reporter assay showed that BcWRKY1 could bind to the promoter of BcZAT12, and BcsAPX expression was activated by BcZAT12. To sum up, we propose a BcWRKY1–BcZAT12–BcsAPX regulatory model that involves in pak-choi salt stress response. |
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