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piRNA-Triggered MIWI Ubiquitination and Removal by APC/C in Late Spermatogenesis
Authors:Shuang Zhao  Lan-Tao Gou  Man Zhang  Li-Dong Zu  Min-Min Hua  Ye Hua  Hui-Juan Shi  Yong Li  Jinsong Li  Dangsheng Li  En-Duo Wang  Mo-Fang Liu
Affiliation:1. State Key Laboratory of Molecular Biology, Graduate University of Chinese Academy of Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;2. Shanghai Key Laboratory of Molecular Andrology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;3. Center for RNA Research, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;4. State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;5. Shanghai Information Center for Life Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China;6. National Population and Family Planning Committee, Key Laboratory of Contraceptive Drugs and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China;7. Department of Biochemistry and Molecular Biology, School of Medicine, University of Louisville, Louisville, KY 40202, USA;8. Center for Genetics and Molecular Medicine, School of Medicine, University of Louisville, Louisville, KY 40202, USA
Abstract:
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  • Highlights? MIWI is a substrate of APC/C, and piRNA loading is essential for MIWI ubiquitination ? piRNA loading promotes MIWI binding to the APC/C substrate-binding subunit ? MIWI and piRNAs are coordinately eliminated in late spermatids ? Inhibition of MIWI destruction in late spermatids prevents sperm maturation
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