茶毛虫核型多角体病毒的血清学特性

用免疫双扩散、对流免疫电泳、酶联免疫吸附试验(ELISA),固相免疫电镜(SPIEM)等技术,对茶毛虫核型多角体病毒(EpNPV)的抗原特性及与其它10种核型多角体病毒的血清学关系进行分析。结果表明,EpNPV粒子的抗血清只能与EpNPV粒子起反应,不与EpNPV的多角体蛋白及其它10种昆虫核型多角体病毒(NPV)粒子发生交叉反应;EpNPV多角体蛋白抗血清除了和其同源的多角体蛋白起反应外,还能和其它两种NPV的多角体蛋白起反应。以上结果说明了EpNPV的结构蛋白具有较高的抗原特异性,而多角体蛋白则没有种间特异性。同时将固相免疫电镜技术应用到昆虫病毒的血清学检测中,取得了较为理想的结果。

Immunity Studies on the Euproctis pseudoconspersa Nuclear Polyhedrosis Virus

Methods such as immunodiffusion. counter-immunoelectrophoresis. enzyme-linked immunosorbent assay (ELISA) and solidphase immune electron microscopic(SPIEM) technique were used to study the antigenicity of EpNPV and relationships between EpNPV and other ten kinds of NPV Results indicated that the antiserum of EpNPV reacted only with the purified particles of EpNPV, and did not produce any immunoreaction with polyhedron protein and other ten kinds of NPV. Such tests suggested that there may be no serological relationships between them. The structural protein of EpNPV has a high specificity, The antiserum of polyhedron protein of EpNPV reacted not only with the polyhedron protein of EpNPV, but also with the polyhedron protein of EoNPV and PINPV. Polyhedron protein of insect baculoviruses has no specificity.