Adipose triglyceride lipase (ATGL) expression in human skeletal muscle is type I (oxidative) fiber specific |
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Authors: | Johan W E Jocken Egbert Smit Gijs H Goossens Yvonne P G Essers Marleen A van Baak Marco Mensink Wim H M Saris Ellen E Blaak |
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Institution: | (1) Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, PO Box 616, 6200 MD Maastricht, The Netherlands |
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Abstract: | Accumulation of triacylglycerol (TAG) and lipid intermediates in skeletal muscle plays an important role in the etiology of
insulin resistance and type 2 diabetes mellitus. Disturbances in skeletal muscle lipid turnover and lipolysis may contribute
significantly to this. So far, knowledge on the regulation of muscle lipolysis is limited. Recently the identification of
a new lipase was reported: adipose triglyceride lipase (ATGL). ATGL deficient animals show significant lipid accumulation
in skeletal muscle, which may indicate that ATGL plays a pivotal role in skeletal muscle lipolysis. However, until now, it
is still unknown whether ATGL protein is expressed in human skeletal muscle. Therefore, the aim of the present study was to
investigate whether ATGL is expressed at the protein level in human skeletal muscle, and to examine whether its expression
is fiber-type specific. To accomplish this, we established an imunohistochemical and immunofluorescent staining procedure
to study ATGL protein expression in relation to fiber type in human vastus lateralis muscle of eight male subjects (BMI range:
21.0–34.5 kg/m2 and age: 38–59 years). In the present paper we report for the first time that ATGL protein is indeed expressed in human skeletal
muscle. Moreover, ATGL is exclusively expressed in type I (oxidative) muscle fibers, suggesting a pivotal role for ATGL in
intramuscular fatty acid handling, lipid storage and breakdown. |
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Keywords: | ATGL Skeletal muscle Fiber type Obesity Protein |
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