Comparison of two methods for callus culture and plant regeneration in wheat (Triticum aestivum) |
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| Authors: | Akbar S. Mohmand Murray W. Nabors |
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| Affiliation: | (1) Department of Biology/Botany, Colorado State University, 80523 Fort Collins, CO, USA;(2) Present address: Tissue Culture Lab, National Agricultural Research Centre, Park Road, P.O. N.I.H., Islamabad, Pakistan |
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| Abstract: | Callus growth and development involve a complex relationship between the explants used to initiate callus, the constituents of the medium and the environmental conditions during culturing. Use of high molecular weight osmotica such as polyethylene glycol (PEG-4000) results in non-solidification of agar medium used for culturing and selection. Thus, a new filter paper bridge technique was compared with the existing agar medium for callus initiation, multiplication, and plant regeneration of wheat. The yield of both total and embryogenic callus was doubled and significantly higher number of regenerants was obtained on filter paper bridges compared to agar medium. |
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| Keywords: | agar medium callus size embryogenic callus filter paper bridges polyethylene glycol |
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