Subcellular distribution of total poly (A) -containing RNA and ferritin-mRNA in the cytoplasm of rat liver. |
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Authors: | Josef Zähringer BS Baliga HN Munro |
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Institution: | Laboratory of Physiological Chemistry Department of Nutrition and Food Science Massachusetts Institute of Technology Cambridge, Massachusetts 02139 USA |
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Abstract: | Poly(A)-containing RNA was isolated from rat liver microsomes and from the post-microsomal supernatant fraction. Approximately 15% of total rat liver poly(A)-containing RNA was found to be present in the post-microsomal supernatant. The relative capacity for apoferritin synthesis of each poly(A)-containing RNA preparation was measured in a cell-free system derived from wheat germ. The post-microsomal supernatant fraction was found to be highly enriched with ferritin mRNA and accounted for 40–50% of the total ferritin-mRNA present in the cytoplasm of rat liver. |
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