Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication |
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| Authors: | Elazar Menashe Cheong Kwang Ho Liu Ping Greenberg Harry B Rice Charles M Glenn Jeffrey S |
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| Institution: | Division of Gastroenterology and Hepatology, Stanford University School of Medicine, Palo Alto, California 94305, USA. |
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| Abstract: | We identified an N-terminal amphipathic helix (AH) in one of hepatitis C virus (HCV)'s nonstructural proteins, NS5A. This AH is necessary and sufficient for membrane localization and is conserved across isolates. Genetically disrupting the AH impairs HCV replication. Moreover, an AH peptide-mimic inhibits the membrane association of NS5A in a dose-dependent manner. These results have exciting implications for the HCV life cycle and novel antiviral strategies. |
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