Use of nfsB, encoding nitroreductase, as a reporter gene to determine the mutational spectrum of spontaneous mutations in Neisseria gonorrhoeae |
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| Authors: | Daniel C Stein Esteban Carrizosa and Stephen Dunham |
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| Institution: | (1) Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA;(2) University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA;(3) Antibacterial Molecular Sciences, Pfizer Global Research and Development, Ann Arbor, MI, USA |
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| Abstract: | Background Organisms that are sensitive to nitrofurantoin express a nitroreductase. Since bacterial resistance to this compound results
primarily from mutations in the gene encoding nitroreductase, the resulting loss of function of nitroreductase results in
a selectable phenotype; resistance to nitrofurantoin. We exploited this direct selection for mutation to study the frequency
at which spontaneous mutations arise (transitions and transversions, insertions and deletions). |
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