| T-DNA插入突变在植物功能基因组学中的应用 |
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| 引用本文： | 赵霞,周波,李玉花.T-DNA插入突变在植物功能基因组学中的应用[J].生物技术通讯,2009,20(6):880-884. |
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| 作者姓名： | 赵霞 周波 李玉花 |
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| 作者单位： | 东北林业大学生命科学学院,黑龙江哈尔滨150040 |
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| 摘 要： | T-DNA插入突变在植物功能基因组学研究中发挥着重要作用,广泛应用于大规模植物基因功能分析,是分离新基因、研究基因功能的有效工具。我们简单介绍了T-DNA插入突变的原理,详细论述了3种T-DNA插入突变载体的应用,并综述了利用T-DNA插入突变克隆新基因的方法,同时指出了T-DNA插入突变存在的问题及其发展方向。
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| 关 键 词： | T-DNA 插入突变 植物 功能基因组学 |
Application of T-DNA Insertion Mutagenesis in Functional Genomics of Plant |
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| ZHAO Xia,ZHOU Bo,LI Yu-Hua.Application of T-DNA Insertion Mutagenesis in Functional Genomics of Plant[J].Letters in Biotechnology,2009,20(6):880-884. |
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| Authors: | ZHAO Xia ZHOU Bo LI Yu-Hua |
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| Institution: | (College of Life Sciences, Northeast Forestry University, Harbin 150040, China) |
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| Abstract: | T-DNA insertion mutagenesis, as an effective tool to separate and study new gene, plays an important role in functional genomics research and widely used in large-scale functional analysis of plant gene. In this review, the recent advances of T-DNA inertion mutagenesis were introduced, including the theory of mutagenesis, the application of three types of vectors, methods for cloning a new gene by T-DNA inertion mutagenesis, and disadvantages and development of T-DNA inertion mutagenesis. |
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| Keywords: | T-DNA |
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