Mutation of Clostridium thermocellum in the presence of certain carbon sources |
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Authors: | Saraswathy V. Nochur Mary F. Roberts Arnold L. Demain |
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Affiliation: | Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 07139, U.S.A.;Francis Bitter National Magnet Laboratory, Massachussetts Institute of Technology, Cambridge, MA 07139, U.S.A. |
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Abstract: | Abstract When a cellobiose-grown inoculum of Clostridium thermocellum was transferred to either glucose or fructose as the sole carbon sourcem growth occurred only after a long lag of 180–200 h. We established that sugar uptake and phosphorylation were not limiting growth nor was the lag period the time take for a physiological adaptation process or for the growth of a mutant carried over in the cellibiose-grown incoculum. It became apparent that a mutation was occuring during the lag period in response to the selection pressure exerted by the presence of glucose or fructose as the sole carbon source. Once growth occurred on glucose and fructose, the cells could be transferred to cellobiose and back to glucose or fructose without exhibiting the long lag period. The change was stable over several transfers in the respective sugars. |
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Keywords: | Clostridium thermocellum Cellulase Directed mutation Growth lag Carbon source |
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