Inhibition of arachidonic acid oxidation by beta-carotene, retinol and alpha-tocopherol

Prostaglandin and hydroxyeicosatetraenoic acid (HETE) production from arachidonic acid in bovine seminal vesicles and kidney as influenced by the addition of beta-carotene, retinol or alpha-tocopherol was studied. The major product formed was prostaglandin E2 (approximately 85% prostaglandin E2 of control), and its proportion decreased with increasing concentration of the additives, while the proportion of HETE increased. Prostaglandin and HETE production was considerably inhibited by beta-carotene and retinol, and to a lesser extent by alpha-tocopherol; HETE production was inhibited less than that of prostaglandin. It appears that beta-carotene, retinol and alpha-tocopherol influence both the cyclooxygenase and lipoxygenase pathways; this modulation of arachidonic acid oxidation by physiological compounds may have important in vivo implications.