Molecular weight determination of random coil polypeptides from collagen by molecular sieve chromatography |
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| Authors: | K A Piez |
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| Affiliation: | 1. Department of Neurosurgery, Beijing Tian Tan Hospital, Capital Medical University, Beijing, China;2. China National Clinical Research Center for Neurological Diseases, China;3. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, China;1. Institute of Public Health, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China;2. Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China;3. Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China |
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| Abstract: | The chromatographic properties on molecular sieve columns of polypeptides from collagen were investigated under conditions in which the polypeptides are random coils. An acrylamide gel (Bio-Gel P-150) was found suitable for the separation and molecular weight determination of polypeptides with molecular weights of 1,500 to 24,000. An 8% agarose gel (Bio-Gel A-1.5) could be used for the molecular weight range 3,000 to 200,000 and gave as good resolution as the acrylamide gel for peptides chromatographing within the inclusion volume of both gels. |
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