Immunochemical differentiation of glucose phosphate isomerase (GPI) allozymes of the mouse |
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| Authors: | U Conrad G Walter D Helbing R Manteuffel J Schöneich |
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| Institution: | Zentralinstitut für Genetik und Kulturpflanzenforschung der AdW der DDR, Gatersleben, German Democratic Republic. |
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| Abstract: | Polyclonal xenoantisera against mouse GPI-1B and GPI-1C were produced in rabbits and analyzed for their ability to recognize allozyme-specific determinants. These studies showed a high degree of serological similarity among the three allozymes of mouse glucose phosphate isomerase (GPI). However, GPI-1B and GPI-1C could be differentiated from GPI-1A as well as GPI-1A and GPI-1B from GPI-1C using quantitative solid-phase immunobinding assays. In addition, polyclonal and monoclonal alloantibodies specific for GPI-1C were produced in BALB/c (Gpi-1a/Gpi-1a) mice. As indicated by immunoblotting data, the allozyme specificity of rabbit antisera and monoclonal alloantibodies against GPI-1C is dependent on the native structure of that allozyme. |
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