Molecular cloning,characterization and expression analysis of a new gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from <Emphasis Type="Italic">Salvia miltiorrhiza</Emphasis> |
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Authors: | Pan Liao Wei Zhou Lin Zhang Jing Wang Xiangming Yan Yan Zhang Ran Zhang Li Li Genyu Zhou Guoyin Kai |
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Institution: | (1) Laboratory of Plant Biotechnology, College of Life and Environment Sciences, Shanghai Normal University, 200234 Shanghai, People’s Republic of China;(2) Department of Pharmacy, Shaoxing People’s Hospital, 312000 Shaoxing, People’s Republic of China |
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Abstract: | The 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) catalyzes the conversion of HMG-CoA to mevalonate (MVA), which is the
first committed step in MVA pathway for isoprenoid biosynthesis in plants. In this study, a full-length cDNA encoding HMGR
was isolated from Salvia miltiorrhiza by rapid amplification of cDNA ends (RACE) for the first time, which was designated as SmHMGR (GenBank Accession No.EU680958). The full-length cDNA of SmHMGR was 2,115 bp containing a 1,695 bp open reading frame (ORF) encoding a polypeptide of 565 amino acids. Bioinformatic analyzes
revealed that the deduced SmHMGR had extensive homology with other plant HMGRs contained two transmembrane domains and a catalytic domain. Molecular modeling
showed that SmHMGR is a new HMGR with a spatial structure similar to other plant HMGRs. Phylogenetic tree analysis indicated
that SmHMGR belongs to the plant HMGR super-family and has the closest relationship with HMGR from Picrorhiza kurrooa. Expression pattern analysis implied that SmHMGR expressed highest in root, followed by stem and leaf. The expression of SmHMGR could be up-regulated by salicylic acid (SA) and methyl jasmonate (MeJA), suggesting that SmHMGR was elicitor-responsive. This work will be helpful to understand more about the role of HMGR involved in the tanshinones
biosynthesis at the molecular level. |
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Keywords: | Expression analysis Molecular cloning Salvia miltiorrhiza SmHMGR Tanshinones |
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