Construction of a Genetic Map and Development of DNA Markers Linked to the Sex-Determining Locus in the Patagonian Pejerrey (Odontesthes hatcheri) |
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Authors: | Eriko Koshimizu Carlos Augusto Strüssmann Nobuaki Okamoto Hideo Fukuda Takashi Sakamoto |
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Institution: | (1) Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato, Tokyo 108-8477, Japan; |
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Abstract: | The process of sex differentiation in fishes is regulated by genetic and environmental factors. The sex of Patagonian pejerrey
(Odontesthes hatcheri) appears to be under strong genotypic control (GSD) because the sex ratios are balanced (1:1) between 17°C and 23°C. However,
sex ratios become female-biased at <15°C and male-biased at 25°C, which shows that this species also possesses some degree
of temperature-dependent sex determination (TSD). Identification of the genetic sex of an individual will help elucidate the
molecular basis of sex differentiation in this species. In this study, we used amplified fragment length polymorphism (AFLP)
analysis to develop a genetic linkage map for both sexes and a sex-linked DNA marker for Patagonian pejerrey. The AFLP analysis
of 23 male and 23 female progeny via 64 primer combinations produced a total of 153 bands. The genetic linkage map consisted
of 79 markers in 20 linkage groups and 48 markers in 15 linkage groups for males and females, respectively. One AFLP marker
tightly linked to the sex-determining locus was identified: the marker, ACG/CAA-217, amplified to the male-specific DNA fragment.
Sequence analysis of this region revealed a single nucleotide polymorphism (SNP) between males and females, which was converted
into a SNP marker. This marker provides genetic confirmation that the sex of Patagonian pejerrey is determined genetically
and would be useful for the analysis of the molecular basis of GSD and TSD in this species. |
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