High-Performance Capillary Electrophoretic Characterization of Different Types of Oligo- and Polysialic Acid Chains |
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Authors: | Mou-Chi Cheng Shu-Ling Lin Shih-Hsiung Wu Sadako Inoue Yasuo Inoue |
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Institution: | Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei, 115, Taiwan |
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Abstract: | We have carried out comparative structural analysis of novel oligo- and polysialic acid chains from diverse sources. Controlled acid hydrolysates of (a) colominic acid, α2→8-linked homopolymer ofN-acetylneuraminic acid (Neu5Ac), (b) α2→8-linked oligo/polyNeu5Gc chains present in rainbow trout egg polysialoglycoprotein, and (c) α2→8-linked oligomers of deaminoneuraminic acid (KDN) residues of KDN-rich glycoprotein derived from rainbow trout vitelline envelope were analyzed by high-performance capillary electrophoresis (HPCE). The results showed that three different types of α2→8-linked oligosialic acids having same degree of polymerization can be separated by HPCE. A partial hydrolysate of colominic acid with mild acid was shown by CE to form intramolecular esters during the controlled hydrolysis and the subsequent workup procedure. In contrast, lactonization of (→5-Oglycolyl-Neu5Gcα2→)n, α2→5-Oglycolyl-linked homopolymer ofN-glycolylneuraminic acid (Neu5Gc) present in the egg jelly coat of sea urchin, did not take place as readily as in (→8Neu5Acα2→)n. |
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Keywords: | oligo/polysialic acids α 2→ 8-linked oligo/polyNeu5Ac oligo/polyNeu5Gc and oligo/polyKDN α 2→ 5-Oglycolyl-linked oligo/polyNeu5Gc capillary electrophoresis |
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