枯草芽孢杆菌群β-甘露聚糖酶基因克隆及同源性分析

本文对33株枯草芽孢杆菌群菌株进行β-甘露聚糖酶活性筛选,其中的32株具有β-甘露聚糖酶活性,只有1株无β-甘露聚糖酶活性.通过基因克隆测序的方法获得33株枯草芽孢杆菌群菌株β-甘露聚糖酶基因编码区全序列,对酶基因进行同源性分析并构建系统发育树;在β-甘露聚糖酶基因系统发育树中,33株枯草芽孢杆菌群菌株聚为3个分支,分别是枯草芽孢杆菌分支、地衣芽孢杆菌分支和解淀粉芽孢杆菌分支;枯草芽孢杆菌、地衣芽孢杆菌和解淀粉芽孢杆菌β-甘露聚糖酶基因种内同源性大于91%,而种间同源性为60%69%.

Cloning and Homology Analysis of Beta-mannanase Gene from the Collection of Bacillus subtilis Group

The beta-mannanase activity of thirty-three strains from the collection of Bacillus subtilis group were determinated in this study,the result revealed that thirty-two tested strains exhibited beta-mannanase activity as well as only one strain's beta-marmanase activity wasn't detected.The complete encoding sequences of beta-mannanase gene from thirty-three B.subtilis group strains were obtained by using gene cloning and sequencing,and the beta-mannanase gene homology was analyzed and a phylogenetic tree was generated.In the phylogenetic tree of the beta-mannanase gene,thirty-three B.subtilis group strains were clustered into three distinct clades,the B.subtilis clade,the B.licheniformis clade and the B.amyloliquefaciens clade.The homology of beta-marmanase genes from B.subtilis,B.licheniformis and B.amyloliquefacieus was above 91% at the intraspcies level,while the homology ranging from about 60% to 69% at the interspecies level,which could provide a gist for the classification and identification of Bacillus subtilis group strains.