Ion movements associated with chorion elevation during egg laying in trout (Oncorhynchus mykiss) in fresh water |
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Authors: | J -P Girard S Gatti-Favereau |
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Institution: | (1) Laboratoire de Physiologie Cellulaire et Comparée, URA CNRS 651, Faculté des Sciences, BP 71, F-06108 Nice Cedex 2, France |
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Abstract: | Within 1 min of transfer from coelomic fluid to fresh water, eggs of rainbow trout (Oncorhynchus mykiss) underwent a transient loss of Na+ and K+ coupled with an elevation of the chorionic envelope. Both mechanisms were blocked by adding a monovalent cation Li+ or K+ (140 mmol·l-1) to the fresh water, but the divalent ion Mg2+ (100 mmol MgCl2·l-1) or elevating the osmotic pressure to 300 mOsmol·l-1 with glycine had no inhibitory effect. The blocking of Na+ loss occurred at external monovalent cation (LiCl) concentrations above 70 mmol·l-1. A 20-s exposure of eggs to fresh water was sufficient to trigger Na+ loss and chorion elevation, even when the eggs were subsequently transferred to fresh water containing 140 mmol LiCl·l-1. Eggs placed in a medium containing 140 mmol LiCl·l-1 and 2 mmol Ca(NO3)2·l-1 showed chorion elevation and associated Na+ loss after addition of calcium ionophore (20 mol·l-1 A.23187). This activation by calcium ionophore was supressed in a Ca2+-free medium containing 5 mmol EGTA·l-1. |
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Keywords: | Ion movements Egg laying Chorion elevation Trout Oncorhynchus mykiss |
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