Combined enzymatic modification of stevioside and rebaudioside A |
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| Authors: | V T Kochikyan A A Markosyan L A Abelyan A M Balayan V A Abelyan |
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| Institution: | (1) Institute of Microbiology, National Academy of Science of Armenia, Abovyan, 375510, Armenia;(2) Biotechnological Corporation Stevian, Kuala Lumpur, 50450, Malaysia |
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| Abstract: | Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by Bacillus stearothermophilus B-5076 B. Macerans BIO-4m were the most effective biocatalysts. This method can be used successfully for direct transglycosylation of stevia extract without purification of its individual components. |
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