Bile acid biotransformation rates of selected gram-positive and gram-negative intestinal anaerobic bacteria. |
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Authors: | P B Hylemon E J Stellwag |
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Institution: | Department of Microbiology Health Sciences Division Virginia Commonwealth University Richmond, Virginia 23298 USA |
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Abstract: | Treatment of whole cell suspensions of and with lysozyme resulted in a marked increase (>100-fold) in the rates of biotransformation of cholate to 7-ketodeoxycholate (7-KD) in the former but only a 2-fold increase in the latter bacterium. In the total activity of both NAD-dependent 7-α-hydroxysteroid dehydrogenase (7-α-OHSDH) and bile salt hydrolase (BSH) increase markedly during the stationary growth phase. Both enzymes were found in the spheroplast lysate and the Triton-soluble washed membrane fractions but only BSH was found in the spheroplast medium. |
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