Activity of antioxidant enzyme during <Emphasis Type="Italic">in vitro</Emphasis> organogenesis in <Emphasis Type="Italic">Crocus sativus</Emphasis> |
| |
| Authors: | E Vatankhah V Niknam H Ebrahimzadeh |
| |
| Institution: | (1) Biotechnology Laboratory, Instituto de Desarrollo Regional, UCLM, Campus Universitario s/n, Albacete, 02071, Spain;(2) Department Plant Nutrition, CEBAS (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain;(3) Department Plant Breeding, CEBAS (CSIC), P.O. Box 164, 30100 Espinardo, Murcia, Spain; |
| |
| Abstract: | The effect of various hormonal combinations on regeneration of shoots and roots from meristem-derived callus of Crocus sativus L. and activities of antioxidant enzymes have been studied. The most efficient regeneration occurred with 1.0 mg dm−3 1-naphthaleneacetic acid (NAA) + 1.0 mg dm−3 thidiazuron and 1.0 mg dm−3 NAA + 2.0 mg dm−3 kinetin. For sprouting, regenerated shoot were subcultured on Murashige and Skoog medium containing 1.0 mg dm−3 NAA + 1.0 mg dm−3 benzylaminopurine (BAP). Protein content and superoxide dismutase activity decreased in regenerated shoots and roots and
increased in sprouting shoots, while catalase (CAT), peroxidase (POX) and polyphenol oxidase (PPO) activities increased during
organogenesis and decreased in sprouting shoots. High CAT and PPO activities were detected in regenerated roots, whereas high
POX activity was observed in regenerated shoot. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
