| 蛋白质组研究中离子阱串联质谱数据搜库结果解释方法 |
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| 引用本文： | 张纪阳,朱云平,谢红卫,贺福初. 蛋白质组研究中离子阱串联质谱数据搜库结果解释方法[J]. 生物物理学报, 2006, 22(4): 244-250 |
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| 作者姓名： | 张纪阳 朱云平 谢红卫 贺福初 |
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| 作者单位： | 1. 国防科学技术大学机电工程与自动化学院,长沙,410073
2. 军事医学科学院放射与辐射医学研究所,北京,100850;北京蛋白质组研究中心,北京,102206 |
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| 摘 要： | 基于离子阱串联质谱仪的鸟枪法是一种高通量的蛋白质鉴定方法。得到的数据一般使用软件SEQUEST搜索蛋白质序列数据库，得到肽段鉴定列表以及相应的打分。为了得到蛋白质鉴定列表，还需要进行肽段鉴定结果的过滤和假阳性率的计算，然后根据肽段鉴定结果组装蛋白质列表。这两个问题目前还没有很好地解决。对已有的方法进行总结和比较，可以给搜库结果解释方法的选择提供参考，对数据质量控制方法的改进也有所帮助。
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| 关 键 词： | 离子阱串联质谱 数据质量控制 蛋白质鉴定 |
| 收稿时间： | 2005-11-24 |
| 修稿时间： | 2005-11-24 |
THE INTERPRETATION METHODS OF DATABASE SEARCH RESULTS IN PROTEOMICS REASEARCH: USING ION TRAP TANDAM MASS SPECTROMETRY |
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| ZHANG Ji-yang,ZHU Yun-ping,XIE Hong-wei,HE Fu-chu. THE INTERPRETATION METHODS OF DATABASE SEARCH RESULTS IN PROTEOMICS REASEARCH: USING ION TRAP TANDAM MASS SPECTROMETRY[J]. Acta Biophysica Sinica, 2006, 22(4): 244-250 |
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| Authors: | ZHANG Ji-yang ZHU Yun-ping XIE Hong-wei HE Fu-chu |
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| Affiliation: | 1. College of Mechanical &|Electronic Engineering and Automatization, National University fo Defense Technology, Changsha 410073, China|2. Beijing Institute of Radiation Medicine, Beijing 100850, China|3. Beijing Proteome Resesarch Center, Beijing 102206, China |
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| Abstract: | Shotgun method based on ion trap tandem mass spectrometry is a high-thought protein identification strategy in proteomic research. The data is usually processed by searching protein sequence database with software SEQUEST. Each spectrum is assigned some peptides with some scores. In order to determine the identified proteins, filteration and false positive rate estimation should be performed on the peptide identifition results, and then the protein list can be inferred. The two problems have no perfect solution currently. Reviewing the method can provide a reference of the strategy selection and be helpful for the data quality control. |
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| Keywords: | SEQUEST |
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