The structures of the serine-linked sugar chains on human chorionic gonadotropin |
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| Authors: | L A Cole S Birken F Perini |
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| Institution: | 1. Department of Internal Medicine, MPB Box 19, University of Michigan Medical School, Ann Arbor, MI 48109 USA;2. Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI 48109 USA;3. Department of Medicine, College of Physicians and Surgeons of Columbia University, New York, NY 10032 USA;1. Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar, Punjab 160062, India;2. Department of Pharmacoinformatics, National Institute of Pharmaceutical Education and Research, Sector 67, S.A.S. Nagar, Punjab 160062, India;1. Adjunct Professor, Department of Developmental Sciences, Marquette University School of Dentistry, Milwaukee, Wis;2. Associate Professor, Department of Orthodontics, Rutgers School of Dental Medicine, Newark, N.J;3. Assistant Professor, Department of Oral Health and Rehabilitation, University of Louisville School of Dentistry, Louisville, Ky;4. Research Associate I, Department of Institutional Assessment and Quality Improvement, Rutgers School of Dental Medicine, Newark, N.J;1. Jenner Institute, University of Oxford and the NIHR Oxford Biomedical Research Centre, Oxford OX3 7DQ, UK;2. Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, Old Road, Headington, Oxford OX3 7LJ, United Kingdom;3. Novavax AB, Kungsgatan 109, SE-753 18 Uppsala, Sweden;1. FMVZ, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-Xmatkuil, Mérida, Yucatán, Mexico;2. INRA UMR 1225 Interactions Hôte Agents Pathogènes, 23 Chemin des Capelles, F31076, Toulouse, France;3. Université de Toulouse, INP-ENSIACET, LCA (Laboratoire de Chimie Agro industrielle), F31030 Toulouse, France |
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| Abstract: | The human chorionic gonadotropin beta-subunit tryptic COOH-terminal peptide (residues 123-145) which contains 3 serine-linked sugar chains was isolated. The sugar chains were cleaved by beta-elimination and then separated by gel filtration. The peaks were pooled and their compositions determined. The products of serial glycosidase digestion and periodate oxidation of the intact glycopeptide were also characterized. Of the serine-linked sugar chains, 13% were the hexasaccharide NeuAc alpha 2,3 Gal beta 1,3 (NeuAc alpha 2,3 Gal beta 1,4 GlcNAc beta 1,6) GalNAc, 34% the tetrasaccharide NeuAc alpha 2,3 Gal beta 1,3 (NeuAc alpha 2,6) GalNAc, 43% the trisaccharide NeuAc alpha 2,3 Gal beta 1,3 GalNAc and 10% the disaccharide NeuAc alpha 2,6 GalNAc. |
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