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| 放线菌15个属中线型染色体和线型质粒的检测 | |
| 引用本文： | 马宁,马伟,姜成林,方萍,覃重军.放线菌15个属中线型染色体和线型质粒的检测[J].微生物学报,2003,43(5):666-670. |
| 作者姓名： | 马宁 马伟 姜成林 方萍 覃重军 |
| 作者单位： | 1. 中国科学院上海植物生理生态研究所,上海,200032 2. 云南大学微生物研究所,云南,650091 3. 同济大学环境科学与工程学院,污染控制与资源化国家重点实验室,上海,200092 |
| 基金项目： | 上海市自然科学基金 (0 2ZA14 0 96)~~ |
| 摘 要： | 在属于放线菌目 (Actinomycetales)链霉菌属 (Streptomyces)下的不同种中发现了约 5 0 0 0种抗生素和生理活性物质 ,其作用包括抗细菌、抗真菌、除草、杀虫、抗肿瘤和免疫抑制剂等1 ] 。在属于放线菌目的红球菌属 (Rhodococcus)和诺卡氏菌属 (Nocardia)中有许多种可以降解多种工业有毒化合物 ,如苯酚、多卤联苯、脂环烃和硝基芳族等2 ] 。一般细菌的染色体和质粒DNA为环型结构。近年来 ,人们利用脉冲电泳技术发现在链霉菌等少数细菌中存在线型结构的染色体和质粒3] ,有的巨大线型质粒上还带有完整的抗生素生物合成基因簇4 ]或降解多… |
| 关 键 词： | 放线菌，线型染色体，线型质粒 |
| 文章编号： | 0001-6209(2003)05-0666-05 |
| 修稿时间： | 2002年11月25 |
Detection of Linear Chromosomes and Plasmids Among 15 Genera in the Actinomycetales |
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| Ma Ning,Ma Wei,Jiang Chenglin,Fang Ping,Qin Zhongjun.Detection of Linear Chromosomes and Plasmids Among 15 Genera in the Actinomycetales[J].Acta Microbiologica Sinica,2003,43(5):666-670. | |
| Authors: | Ma Ning Ma Wei Jiang Chenglin Fang Ping Qin Zhongjun |
| Institution: | Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China. nma@iris.sipp.ac.cn |
| Abstract: | Bacterial chromosomes and plasmids are commonly circular, however, lin ear chromosomes and plasmids were discovered among 5 genera of the Actinomycet ales. Here, we use pulsed field gel electrophoresis to study the genomes of 19 spe cies which belong to 15 genera in the Actinomycetales. All chromosomes of 19 spe cies are linear DNA, and linear plasmids with different sizes and copy numbers a re detected among 5 species. This work provide basis for investigating the possi ble novel functions of linear replicons beyond Streptomyces and also helps t o develop Actinomycetales artificial linear chromosome. |
| Keywords: | Actinomycetales Linear chromosomes Linear plasmids |
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