Genotyping accuracy for whole-genome amplification of DNA from buccal epithelial cells. |
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| Authors: | Keren Leviel Megan Olarte Patrick F Sullivan |
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| Affiliation: | Department of Genetics, University of North Carolina at Chapel Hill, 27599-7264, USA. |
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| Abstract: | We compared the accuracy of genotyping for DNA extracted from lymphocytes to that of DNA amplified from buccal epithelial cells. Amplification was via a rolling circle/phi29 DNA polymerase commercial kit. Paired buccal and lymphocyte DNA samples were available from 30 individuals. All samples were genotyped for 12 SNPs, 5 microsatellites and 2 VNTRs. The accuracy of genotyping (no-call proportions, reproducibility, and concordance) was similar for DNA from lymphocytes in comparison to amplified DNA from buccal samples. If used with caution, these data suggest that rolling-circle whole-genome amplification can be used to increase the DNA mass available for large-scale genotyping projects based on DNA from buccal cells. |
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