Proton-dependent transporter gene <Emphasis Type="Italic">lndJ</Emphasis> confers resistance to landomycin E in <Emphasis Type="Italic">Streptomyces globisporus</Emphasis> 1912 |
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| Authors: | I Ostash B Ostash S Walker V Fedorenko |
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| Institution: | (1) Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, 79005 Lviv, Ukraine;(2) Department of Microbiology and Molecular Genetics, Harvard Medical School, 02115 Boston, USA |
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| Abstract: | Sequence analysis of 2 kb BamHI-SmaI fragment of landomycin E (LaE) gene cluster in Streptomyces globisporus 1912 revealed one complete ORF marked as lndJ. Analysis of putative lndJ aminoacid sequence allowed us to suppose that it is proton-dependent antiporter which could be involved in resistance to
LaE in the producing strain. Although disruption of lndJ had no significant influence on LaE production and resistance, its overexpression in wild type and LaE overproducing strains
led to qualitative changes in landomycins biosynthesis and increased resistance to LaE. These data support the hypothesis
about involvement of lndJ gene in landomycins export.
The text was submitted by the authors in English. |
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