A new chromogenic substrate for assay and detection of alpha-amylase |
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| Authors: | P Biely D Mislovicová O Markovic V Kalác |
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| Affiliation: | Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Czechoslovakia. |
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| Abstract: | A new soluble chromogenic substrate for alpha-amylase was prepared by coupling partially hydrolyzed starch with a dye, Ostazin brilliant red H-3B. The substrate is precipitable from buffered solutions with ethanol and is equally suitable for assay of alpha-amylase, detection of separated alpha-amylase isoenzymes in gels, and selection of microbial producers of the enzyme. |
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