促进原核表达蛋白可溶性的研究进展

以大肠杆菌为代表的原核表达蛋白系统具有操作简单、周期短、成本低、表达量高等优点而成为获得外源表达蛋白的首选方案。但外源蛋白在原核宿主中往往以无生物活性的包涵体形式存在,这限制了原核表达系统的广泛应用。随着对蛋白折叠动力学、参与蛋白折叠的酶和分子伴侣等认识的不断深入,科学家们通过诱导条件优化、宿主细胞改造以及使用融合标签等手段,在促进原核表达蛋白可溶性方面取得了较大的进展。就这些研究进展进行综述,旨在为设计和获得原核可溶表达蛋白的实验方案提供参考。

Advances in Promoting Solubility of Prokaryotic Expressed Proteins

The prokaryotic expression protein system,especially Escherichia coli expression system,is a preferred method for obtaining exogenous proteins because of its advantages of simple operation,short cycle,high expression level and relatively low cost.However,one of the major factors to limit the extensive application of E.coli expression protein system is that the exogenous proteins,when expressed in a high amount,are often present in an insoluble form.With the understanding of protein folding dynamics,enzymes and molecular chaperones involved in protein folding,scientists have made great progress in promoting solubility of heterologous proteins by changing expression conditions,modifying host cells or using fusion tags.Here,we review the related progress in order to provide reference for related research.